multiphoton imaging of peripheral immune cells
A type of intravital microscopy* which involves the optical sectioning of a tissue sample using laser-based–femtosecond-in-duration–pulses of light.
Multiphoton excitation is divided into two-photon and three-photon phenomena, depending on the number of photons that converge to excite the fluorophores, which means that the laser wavelength used for multiphoton excitation is approximately double the wavelength of single-photon excitation used in confocal microscopy. In contrast to confocal microscopy, multiphoton microscopy images deeper tissue planes and has a lower phototoxic potential.
*The imaging of cellular and/or molecular processes within intact tissues in live animals
Reference Immunology and Cell Biology (2013) 91, 263–270